Molecular Recognition and Mechanism of Action of Hammerhead Ribozyme-Metalloenzyme
نویسندگان
چکیده
منابع مشابه
A tale in molecular recognition: the hammerhead ribozyme.
A new crystal structure of the hammerhead ribozyme demonstrates the influence of peripheral tertiary contacts on the local conformations around the active site. This structure resolves many conflicting results obtained on reduced systems.
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پژوهش حاضر با هدف بررسی اثر جیره حاوی کربوهیدراتهای غیرالیافی زیاد و بروز التهاب در گاوهای شیرده در دو آزمایش جداگانه انجام شد. در آزمایش اول 20 رأس گاو مبتلا به ورم پستان از دو گروه کم تولید و پرتولید (هر کدام 10 رأس) و 20 رأس گاو سالم از دو گروه کم تولید و پرتولید (هر کدام 10 رأس) انتخاب شدند. همه گاوها در دوره دوم و یا سوم شیردهی و در روزهای 80-120 پس از زایش بودند. میانگین تولید شیر در گروه...
Role of Mg2+ in hammerhead ribozyme catalysis from molecular simulation.
Molecular dynamics simulations have been performed to investigate the role of Mg2+ in the full-length hammerhead ribozyme cleavage reaction. In particular, the aim of this work is to characterize the binding mode and conformational events that give rise to catalytically active conformations and stabilization of the transition state. Toward this end, a series of eight 12 ns molecular dynamics si...
متن کاملThe hammerhead ribozyme
The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme stimulates nearly complete cleavage of the phosphodiester chain at a defined internal site to give 2',3'-cyclic and 5'-hydroxy termini. The cleavage rate is approx. 1 min-' at 25 "C, pH 7.5, and increases proportionally with hydroxide ion co...
متن کاملThe ubiquitous hammerhead ribozyme.
The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self-scission under physiological conditions. Originally discovered in subviral plant pathogens, its presence in several eukaryotic genomes has been rep...
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ژورنال
عنوان ژورنال: Journal of Japan Oil Chemists' Society
سال: 1994
ISSN: 1884-2003
DOI: 10.5650/jos1956.43.975